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cd4+ t cells isolation protocol  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc cd4+ t cells isolation protocol
    Poly1 particles increase MHCI-mediated antigen presentation and antigen-specific T cell proliferation. (A) MFI of signal pertaining to SIINFEKL presented in MHCI following treatment of DCs with no SIINFEKL (“Untreated”, gray), buffer with SIINFEKL (“SIIN Only”, gray), free Poly1 with SIINFEKL (blue), or Poly1 particles with SIINFEKL (red) and gated under DAPI− cells. 48 hours after treatment of primary DCs as in (A) but with 0.25ug mL−1 of a different model antigen, MOG35-55,CD4+ cells isolated from <t>2D2</t> mice with T cell receptors specific to MOG35-55 were added to the wells and incubated for 72 hours. (B) Representative flow cytometry traces showing CFSE dilutions after treatment with MOG35-55 and either buffer (“MOG Only”, gray), TLR4a LPS (“TLR4”, black), free Poly1 (“Free”, blue), and Poly1 particles formed (“Particles”, red). (C) MFI of CFSE signal among DAPI−/CD3+/CD4+ cells following treatments as in (B). (D) Percentage of DAPI−/CD3+/CD4+ cells proliferated beyond the 2nd generation following treatment as in (B) as determined by CFSE dilution. Samples were prepared in triplicated and errors bars represent SEM.
    Cd4+ T Cells Isolation Protocol, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd4+ t cells isolation protocol/product/STEMCELL Technologies Inc
    Average 90 stars, based on 1 article reviews
    cd4+ t cells isolation protocol - by Bioz Stars, 2026-05
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    Images

    1) Product Images from "Intrinsic immunogenicity of rapidly-degradable polymers evolves during degradation"

    Article Title: Intrinsic immunogenicity of rapidly-degradable polymers evolves during degradation

    Journal: Acta biomaterialia

    doi: 10.1016/j.actbio.2015.12.026

    Poly1 particles increase MHCI-mediated antigen presentation and antigen-specific T cell proliferation. (A) MFI of signal pertaining to SIINFEKL presented in MHCI following treatment of DCs with no SIINFEKL (“Untreated”, gray), buffer with SIINFEKL (“SIIN Only”, gray), free Poly1 with SIINFEKL (blue), or Poly1 particles with SIINFEKL (red) and gated under DAPI− cells. 48 hours after treatment of primary DCs as in (A) but with 0.25ug mL−1 of a different model antigen, MOG35-55,CD4+ cells isolated from 2D2 mice with T cell receptors specific to MOG35-55 were added to the wells and incubated for 72 hours. (B) Representative flow cytometry traces showing CFSE dilutions after treatment with MOG35-55 and either buffer (“MOG Only”, gray), TLR4a LPS (“TLR4”, black), free Poly1 (“Free”, blue), and Poly1 particles formed (“Particles”, red). (C) MFI of CFSE signal among DAPI−/CD3+/CD4+ cells following treatments as in (B). (D) Percentage of DAPI−/CD3+/CD4+ cells proliferated beyond the 2nd generation following treatment as in (B) as determined by CFSE dilution. Samples were prepared in triplicated and errors bars represent SEM.
    Figure Legend Snippet: Poly1 particles increase MHCI-mediated antigen presentation and antigen-specific T cell proliferation. (A) MFI of signal pertaining to SIINFEKL presented in MHCI following treatment of DCs with no SIINFEKL (“Untreated”, gray), buffer with SIINFEKL (“SIIN Only”, gray), free Poly1 with SIINFEKL (blue), or Poly1 particles with SIINFEKL (red) and gated under DAPI− cells. 48 hours after treatment of primary DCs as in (A) but with 0.25ug mL−1 of a different model antigen, MOG35-55,CD4+ cells isolated from 2D2 mice with T cell receptors specific to MOG35-55 were added to the wells and incubated for 72 hours. (B) Representative flow cytometry traces showing CFSE dilutions after treatment with MOG35-55 and either buffer (“MOG Only”, gray), TLR4a LPS (“TLR4”, black), free Poly1 (“Free”, blue), and Poly1 particles formed (“Particles”, red). (C) MFI of CFSE signal among DAPI−/CD3+/CD4+ cells following treatments as in (B). (D) Percentage of DAPI−/CD3+/CD4+ cells proliferated beyond the 2nd generation following treatment as in (B) as determined by CFSE dilution. Samples were prepared in triplicated and errors bars represent SEM.

    Techniques Used: Immunopeptidomics, Isolation, Incubation, Flow Cytometry



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    cd4+ t cells isolation protocol  (STEMCELL Technologies Inc)
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    STEMCELL Technologies Inc cd4+ t cells isolation protocol
    Poly1 particles increase MHCI-mediated antigen presentation and antigen-specific T cell proliferation. (A) MFI of signal pertaining to SIINFEKL presented in MHCI following treatment of DCs with no SIINFEKL (“Untreated”, gray), buffer with SIINFEKL (“SIIN Only”, gray), free Poly1 with SIINFEKL (blue), or Poly1 particles with SIINFEKL (red) and gated under DAPI− cells. 48 hours after treatment of primary DCs as in (A) but with 0.25ug mL−1 of a different model antigen, MOG35-55,CD4+ cells isolated from <t>2D2</t> mice with T cell receptors specific to MOG35-55 were added to the wells and incubated for 72 hours. (B) Representative flow cytometry traces showing CFSE dilutions after treatment with MOG35-55 and either buffer (“MOG Only”, gray), TLR4a LPS (“TLR4”, black), free Poly1 (“Free”, blue), and Poly1 particles formed (“Particles”, red). (C) MFI of CFSE signal among DAPI−/CD3+/CD4+ cells following treatments as in (B). (D) Percentage of DAPI−/CD3+/CD4+ cells proliferated beyond the 2nd generation following treatment as in (B) as determined by CFSE dilution. Samples were prepared in triplicated and errors bars represent SEM.
    Cd4+ T Cells Isolation Protocol, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd4+ t cells isolation protocol/product/STEMCELL Technologies Inc
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    Image Search Results


    Poly1 particles increase MHCI-mediated antigen presentation and antigen-specific T cell proliferation. (A) MFI of signal pertaining to SIINFEKL presented in MHCI following treatment of DCs with no SIINFEKL (“Untreated”, gray), buffer with SIINFEKL (“SIIN Only”, gray), free Poly1 with SIINFEKL (blue), or Poly1 particles with SIINFEKL (red) and gated under DAPI− cells. 48 hours after treatment of primary DCs as in (A) but with 0.25ug mL−1 of a different model antigen, MOG35-55,CD4+ cells isolated from 2D2 mice with T cell receptors specific to MOG35-55 were added to the wells and incubated for 72 hours. (B) Representative flow cytometry traces showing CFSE dilutions after treatment with MOG35-55 and either buffer (“MOG Only”, gray), TLR4a LPS (“TLR4”, black), free Poly1 (“Free”, blue), and Poly1 particles formed (“Particles”, red). (C) MFI of CFSE signal among DAPI−/CD3+/CD4+ cells following treatments as in (B). (D) Percentage of DAPI−/CD3+/CD4+ cells proliferated beyond the 2nd generation following treatment as in (B) as determined by CFSE dilution. Samples were prepared in triplicated and errors bars represent SEM.

    Journal: Acta biomaterialia

    Article Title: Intrinsic immunogenicity of rapidly-degradable polymers evolves during degradation

    doi: 10.1016/j.actbio.2015.12.026

    Figure Lengend Snippet: Poly1 particles increase MHCI-mediated antigen presentation and antigen-specific T cell proliferation. (A) MFI of signal pertaining to SIINFEKL presented in MHCI following treatment of DCs with no SIINFEKL (“Untreated”, gray), buffer with SIINFEKL (“SIIN Only”, gray), free Poly1 with SIINFEKL (blue), or Poly1 particles with SIINFEKL (red) and gated under DAPI− cells. 48 hours after treatment of primary DCs as in (A) but with 0.25ug mL−1 of a different model antigen, MOG35-55,CD4+ cells isolated from 2D2 mice with T cell receptors specific to MOG35-55 were added to the wells and incubated for 72 hours. (B) Representative flow cytometry traces showing CFSE dilutions after treatment with MOG35-55 and either buffer (“MOG Only”, gray), TLR4a LPS (“TLR4”, black), free Poly1 (“Free”, blue), and Poly1 particles formed (“Particles”, red). (C) MFI of CFSE signal among DAPI−/CD3+/CD4+ cells following treatments as in (B). (D) Percentage of DAPI−/CD3+/CD4+ cells proliferated beyond the 2nd generation following treatment as in (B) as determined by CFSE dilution. Samples were prepared in triplicated and errors bars represent SEM.

    Article Snippet: After 48 hours in culture, CD4 + T cells were isolated from the spleens of 2D2 mice using the manufacturer’s recommended protocol (STEMCELL).

    Techniques: Immunopeptidomics, Isolation, Incubation, Flow Cytometry